|Application ||WB, IHC, IF, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||67568 Da|
|Positive Control||WB: HepG2 cell lysate|
|Application & Usage||WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; E 1:5000|
|Other Names||Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90|
|Handling||The antibody solution should be gently mixed before use|
|Reconstitution & Storage||-20°C|
|Precautions||Phospho-Calnexin (Ser583) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor- mediated endocytosis at the synapse.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endoplasmic reticulum. Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545, PubMed:17081065). The palmitoylated form preferentially localizes to the perinuclear rough ER (PubMed:22314232).|
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