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> home > Products > Primary Antibodies > Antibody Collections > Anti-Wnt-Pathway (plus Lgr5) Antibodies > Anti-SMAD5 Antibody (4B10-B10-B6)
Anti-SMAD5 Antibody (4B10-B10-B6)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, FC |
|---|---|
| Primary Accession | Q99717 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone Names | 4B10-B10-B6 |
| Calculated MW | 52258 Da |
| Gene ID | 4090 |
|---|---|
| Application & Usage | WB: Hela, Jurkat and K562 cell lysates; IF: HeLa cells; FC: Jurkat cells |
| Other Names | Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, Smad5, hSmad5 |
| Target/Specificity | SMAD5 |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | In buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | Anti-SMAD5 Antibody (4B10-B10-B6) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SMAD5 |
|---|---|
| Synonyms | MADH5 |
| Function | Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis (PubMed:16516194, PubMed:12064918). Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form an heteromeric complex which translocates into the nucleus acting as transcription factor (PubMed:9442019). In turn, the hetero-trimeric complex recognizes cis- regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network (PubMed:33510867). Non- phosphorylated SMAD5 has a cytoplasmic role in energy metabolism regulation by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes (PubMed:28675158). Mechanistically, interacts with hexokinase 1/HK1 and thereby accelerates glycolysis (PubMed:28675158). |
| Cellular Location | Cytoplasm. Nucleus Mitochondrion. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 |
| Tissue Location | Ubiquitous. |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
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