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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-WDR77 Antibody (8A10-C10-E8)   

Anti-WDR77 Antibody (8A10-C10-E8)

Mouse Monoclonal Antibody

     
  • WB - Anti-WDR77 Antibody (8A10-C10-E8) ABV12071-100
    Western blot detection of WDR77 in C6, 3T3 and K562 cell lysates using WDR77 Antibody
    detail
  • ICC - Anti-WDR77 Antibody (8A10-C10-E8) ABV12071-100
    Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-BiP/GRP78 (C-terminus) Antibody
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession Q9BQA1
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 8A10-C10-E8
Calculated MW 36724 Da
Additional Information
Gene ID 79084
Application & Usage WB; C6, 3T3 and K562 cell lysates, IF: HeLa cells
Other Names Methylosome protein 50, MEP-50, Androgen receptor cofactor p44, WD repeat-containing protein 77, p44/Mep50, MEP50, WD45
Target/Specificity Methylosome protein 50
Antibody Form Liquid
Appearance Colorless liquid
Formulation In PBS (pH 7.4) containing with 0.02% sodium azide and 50% glycerol
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsAnti-WDR77 Antibody (8A10-C10-E8) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WDR77 (HGNC:29652)
Function Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones (PubMed:11756452). This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (PubMed:23071334).
Cellular Location Nucleus. Cytoplasm. Note=Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors (PubMed:17437848).
Tissue Location Highly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors.
Research Areas
Citations (0)
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Background

Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.

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Discontinued
Cat# ABV12071-100
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