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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-DCTN1 Antibody (3D5-C6-D5)   

Anti-DCTN1 Antibody (3D5-C6-D5)

Mouse Monoclonal Antibody

     
  • WB - Anti-DCTN1 Antibody (3D5-C6-D5) ABV12072-100
    Western blot detection of Dynactin 1 in K582, MCF7, 293T and HeLa cell lysates using DCTN1 Antibody
    detail
  • IP - Anti-DCTN1 Antibody (3D5-C6-D5) ABV12072-100
    Immunoprecipitation analysis of HeLa cel! lysates using DCTN1 Antibody
    detail
  • SPECIFICATION
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q14203
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 3D5-C6-D5
Calculated MW 141695 Da
Additional Information
Gene ID 1639
Application & Usage WB: K562, MCF7, 293T and HeLa cell lysates, IP: HeLa cells
Other Names Dynactin subunit 1, 150 kDa dynein-associated polypeptide, DAP-150, DP-150, p135, p150-glued
Target/Specificity Dynactin subunit 1
Antibody Form Liquid
Appearance Colorless liquid
Formulation In PBS (pH 7.4) containing with 0.02% sodium azide and 50% glycerol
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsAnti-DCTN1 Antibody (3D5-C6-D5) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DCTN1 (HGNC:2711)
Function Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity). Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule (PubMed:25185702). Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon (PubMed:23874158). Plays a role in metaphase spindle orientation (PubMed:22327364). Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitment to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole (PubMed:23386061). Plays a role in primary cilia formation (PubMed:25774020).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, spindle. Nucleus envelope. Cytoplasm, cell cortex. Note=Localizes to microtubule plus ends (PubMed:17828277, PubMed:22777741, PubMed:25774020). Localizes preferentially to the ends of tyrosinated microtubules (PubMed:26972003). Localization at centrosome is regulated by SLK- dependent phosphorylation (PubMed:23985322). Localizes to centrosome in a PARKDA-dependent manner (PubMed:20719959). Localizes to the subdistal appendage region of the centriole in a KIF3A-dependent manner (PubMed:23386061). PLK1-mediated phosphorylation at Ser-179 is essential for its localization in the nuclear envelope (PubMed:20679239).
Tissue Location Brain.
Research Areas
Citations (0)
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Background

Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule.

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Discontinued
Cat# ABV12072-100
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