|Calculated MW||43593 Da|
|Positive Control||Western blot|
|Application & Usage||Western blot: 1-4 µg|
|Other Names||Alanine Racemase Y354N, alr, dal|
|Reconstitution & Storage||-20 °C|
|Precautions||Anti-Alanine Racemase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the interconversion of L-alanine and D- alanine. Also weakly active on serine.|
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Provided below are standard protocols that you may find useful for product applications.
Mutant Alanine Racemase (Y354N) (mAR-Y354N), a pyridoxal 5- phosphate (PLP) dependent enzyme catalyzes the interconversion of the L-Serine to D-Serine. In WT Alanine Racemase Tyr354 plays a crucial role in defining the strict specificity of AR for alanine, in converting L-Alanine to D-Alanine, which is an important component of the peptidoglycan layer of bacterial cell wall. By mutating the active site Tyr 354 to Asn, the specificity of the enzyme changes and it becomes a racemase with dual specificity for L- Alanine and L-Serine.
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