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ER alpha monoclonal antibody

Purified Mouse Monoclonal Antibody

     
  • CHIP - ER alpha monoclonal antibody ADN10009
    ChIP using the monoclonal antibody
    ChIP using the monoclonal antibody against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the monoclonal antibody directed against ER alpha and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene, used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.
  • WB - ER alpha monoclonal antibody ADN10009
    Western blot analysis using the monoclonal antibody against hERα
    Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the monoclonal antibody directed against ER alpha at a concentration of 7 µg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.
  • ICC - ER alpha monoclonal antibody ADN10009
    Immunocytochemistry using the monoclonal antibody against hERα
    COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the antibody against ER alpha, used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P03372
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW 66216 Da
Additional Information
Gene ID 2099
Other Names Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, ESR1, ESR, NR3A1
Target/Specificity ER alpha
PrecautionsER alpha monoclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ESR1
Function Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA- binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF- kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA- binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.
Cellular Location Isoform 1: Nucleus {ECO:0000255|PROSITE- ProRule:PRU00407, ECO:0000269|PubMed:12682286}. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=A minor fraction is associated with the inner membrane Nucleus. Golgi apparatus. Cell membrane. Note=Colocalizes with ZDHHC7 and ZDHHC21 in the Golgi apparatus where most probably palmitoylation occurs. Associated with the plasma membrane when palmitoylated
Tissue Location Widely expressed. Isoform 3 is not expressed in the pituitary gland.
Research Areas
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Background

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA- binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF- kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA- binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.

References

Green S.,et al.Nature 320:134-139(1986).
Greene G.L.,et al.Science 231:1150-1154(1986).
Pink J.J.,et al.Nucleic Acids Res. 24:962-969(1996).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Wang Z.,et al.Biochem. Biophys. Res. Commun. 336:1023-1027(2005).

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$ 290.00
Cat# ADN10009
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