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GR monoclonal antibody

Purified Mouse Monoclonal Antibody

  • CHIP - GR monoclonal antibody ADN10010
    ChIP results obtained with the monoclonal antibody directed against hGR
    ChIP assays were performed using HeLa cells, the monoclonal antibody directed against GR and optimized PCR primer sets for qPCR. The cells were treated either with ethanol (EtOH, used as a negative control) or triamcinolone acetonide (TA) for 4 hours prior to cell harvesting. ChIP was performed using sheared chromatin from 3 million cells and 5 µg of antibody. QPCR was performed with primers for the human metallothionein promoter (hMTIIA) and for exon 2 of the human myoglobin gene (hmyo ex2), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the human metallothionein IIA promoter by GR.
  • E - GR monoclonal antibody ADN10010
    Sandwich ELISA
    The specificity of the monoclonal antibody directed against hGR was assessed by sandwich ELISA. Figure 3A: schematic representation of the sandwich ELISA with the monoclonal antibody against hGR . Figure 3B: ELISA results using the monoclonal antibody against hGR at a concentration of 0.5 µg/ml. The figure shows an ELISA signal which is proportionally increasing with increasing amounts of recombinant hGR.
  • WB - GR monoclonal antibody ADN10010
    Western blot analysis using the monoclonal antibody against hGR
    Extracts from HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), and from 5x10e6 Raji or Molt cells were analysed by Western blot using the monoclonal antibody against hGR. Figure 4B. Western blot analysis of extracts from 300,000 HeLa cells with the monoclonal antibody against hGR (concentration 1 µg/ml).
  • IP - GR monoclonal antibody ADN10010
    Immunoprecipitation using the monoclonal antibody against hGR
    The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 µl IP reaction solution) using 5 µg of the monoclonal antibody directed against hGR (Cat. No. MAb-010-050). The IP was followed by Western blot analysis as described above
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04150
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Calculated MW 85659 Da
Additional Information
Gene ID 2908
Other Names Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1, NR3C1, GRL
Target/Specificity GR
PrecautionsGR monoclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NR3C1
Function Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic genes expression.
Cellular Location Cytoplasm. Mitochondrion. Nucleus Note=Cytoplasmic in the absence of ligand, nuclear after ligand- binding
Tissue Location Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
Research Areas
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$ 290.00
Cat# ADN10010
(40 western blots)
Availability: 2-3 days
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