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H3K4me1 monoclonal antibody

Purified Mouse Monoclonal Antibody

     
  • CHIP - H3K4me1 monoclonal antibody ADN10017
    ChIP assays were performed using HeLa cells, the monoclonal antibody against H3K4me1 and optimized PCR primer sets for qPCR. Chromatin was sheared with the Bioruptor using the “Shearing ChIP” kit. ChIP was performed with the “OneDay ChIP” kit , using sheared chromatin from 1.6 million cells. A titration of the antibody consisting of 1, 5 and 10 µg per ChIP experiment was analysed. IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
  • E - H3K4me1 monoclonal antibody ADN10017
    To test the specificity an ELISA was performed using a serial dilution of the monoclonal antibody against H3K4me1. The wells were coated with peptides containing the unmodified H3K4 as well as the mono-, di- and trimethylated H3K4 and the monomethylated H3K9. Figure 2 shows a high specificity of the antibody for the modification of interest.
  • WB - H3K4me1 monoclonal antibody ADN10017
    Histone extracts (15 µg) from HeLa cells were analysed by Western blot using the monoclonal antibody against H3K4me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
  • IF - H3K4me1 monoclonal antibody ADN10017
    HeLa cells were stained with the antibody against H3K4me1 and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K4me1 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P68431
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW 15404 Da
Additional Information
Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968
Other Names Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, H3FA
Target/Specificity H3K4me1
PrecautionsH3K4me1 monoclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HIST1H3A
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Citations (0)

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Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

References

Zhong R.,et al.Nucleic Acids Res. 11:7409-7425(1983).
Marashi F.,et al.Biochem. Cell Biol. 64:277-289(1986).
Albig W.,et al.Genomics 10:940-948(1991).
Kardalinou E.,et al.J. Cell. Biochem. 52:375-383(1993).
Runge D.,et al.Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases.

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$ 290.00
Cat# ADN10017
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