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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Spt16 polyclonal antibody   

Spt16 polyclonal antibody

Purified Rabbit Polyclonal Antibody

     
  • WB - Spt16 polyclonal antibody ADN10240
    Western blot analysis using the antibody directed against SPT16
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9Y5B9
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 119914 Da
Additional Information
Gene ID 11198
Other Names FACT complex subunit SPT16, Chromatin-specific transcription elongation factor 140 kDa subunit, FACT 140 kDa subunit, FACTp140, Facilitates chromatin transcription complex subunit SPT16, hSPT16, SUPT16H, FACT140, FACTP140
Target/Specificity Spt16
PrecautionsSpt16 polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SUPT16H
Function Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II).
Cellular Location Nucleus. Chromosome. Note=Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci
Tissue Location Ubiquitous..
Research Areas
Citations (0)

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Background

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene.

References

Orphanides G.,et al.Nature 400:284-288(1999).
Bienvenut W.V.,et al.Submitted (JUL-2007) to UniProtKB.
Bienvenut W.V.,et al.Submitted (MAR-2008) to UniProtKB.
Kang S.-W.,et al.Genes Cells 5:251-263(2000).
Orphanides G.,et al.Cell 92:105-116(1998).

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$ 190.00
Cat# ADN10240
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