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Functional APRIL (mouse) Antibody, mAb (recombinant) (blocking)

  • E - Functional APRIL (mouse) Antibody, mAb (recombinant) (blocking) ADP0001
    Binding of APRIL (mouse) to BCMA is inhibited by anti-APRIL (mouse), mAb (rec.) (blocking) (APRY-1-1).
    BCMA:Fc was coated on an ELISA plate at 1ug /ml. anti-APRIL (mouse) mAb (rec.) (blocking) (APRY-1-1) or an unrelated mAb (recombinant) (Negative control) were added (starting at 50ug/ml with a twofold serial dilution) together with 0.1ug /ml of MegaAPRIL (mouse). After incubation for 1 h at RT, the MegaAPRIL (mouse) binding was detected using an anti-FLAG antibody (HRP). The percentage of binding is shown.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9D777
Reactivity Mouse
Host Purified From HEK 293 Cell culture Supernatant.
Clonality Monoclonal
Isotype Mouse IgG2bλ
Gene Source Mouse
Endotoxin <0.01EU/ µg purified protein (LAL-test; Lonza)
Application Note E,IP(1:200),Functional Applicational, Inhibits the binding of mouse APRIL to BCMA and TACI.
Clone Names Apry-1-1
Calculated MW 26889 Da
Description APRIL (mouse) monoclonal antibody (recombinant) (blocking) (APRY-1-1) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the mouse lgG2b Fc domain.
Additional Information
Gene ID 69583
Other Names A Proliferation Inducing Ligand; TNFSF13; CD256; TALL-2
Target/Specificity Recognizes mouse APRIL. Does not recognize mouse BAFF.
Format Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Reconstitution & Storage Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
PrecautionsFunctional APRIL (mouse) Antibody, mAb (recombinant) (blocking) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Tnfsf13
Synonyms April
Function Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
Cellular Location Secreted.
Research Areas
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APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth and is involved in monocyte/macrophage-mediated immunological processes.

Anti-APRIL (mouse) Monoclonal Antibody (recombinant) (Blocking) (APRY-1-1) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

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$ 380.00
Cat# ADP0001
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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