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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Functional Antibodies   >   Functional Angiopoietin-2 (human) Antibody, mAb (recombinant) (blocking)   

Functional Angiopoietin-2 (human) Antibody, mAb (recombinant) (blocking)

  • E - Functional Angiopoietin-2 (human) Antibody, mAb (recombinant) (blocking) ADP0004
    Binding of human Angiopoietin-2 to Tie-2 (human):Fc is inhibited by the antibody anti-Angiopoietin-2 (human), mAb (rec.) (blocking) (Angy-1-4).
    Methods:Tie-2 (human):Fc was coated on an ELISA plate at 1 µg/ml. anti-Angiopoietin-2 (human), mAb (rec.) (blocking), (Angy-1-4) or an unrelated mAb (recombinant) (Control) were added (starting at 40 µg/ml with a twofold serial dilution) together with 200ng/ µl of Angiopoietin-2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O15123
Reactivity Human
Host CHO Cells
Clonality Monoclonal
Isotype Human IgG2λ.
Gene Source Human
Application Note ,E,Functional Applicational, Inhibits the binding of human angiopoietin-2 to human Tie-2. ND50= 600-800ng/ml (for 10ng/ml of Angiopoietin-2),ND50 50% neutralizing dose of antibody for a given concentration of ligand (here human Angiopoietin-2).
Clone Names Angy-1-4
Calculated MW 56919 Da
Description anti-Angiopoietin-2 (human), mAb (rec.) (blocking) (Angy-1-4) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain.
Additional Information
Gene ID 285
Other Names Ang-2; Ang2; Angpt2; Agpt2
Target/Specificity Recognizes human angiopoietin-2. Does not recognize mouse angiopoietin-2 and human angiopoietin-1.
Format Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Reconstitution & Storage Stable for at least 1 year after receipt when stored at -20°C.
PrecautionsFunctional Angiopoietin-2 (human) Antibody, mAb (recombinant) (blocking) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
Cellular Location Secreted.
Research Areas
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Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are closely related secreted ligands which bind with similar affinity to Tie-2. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature. Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization. Ang-2 has been identified as a secreted protein ligand of Tie-2 and has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context.

anti-Angiopoietin-2 (human), mAb (rec.) (blocking) (Angy-1-4) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

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$ 350.00
Cat# ADP0004
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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