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Functional IL-33 (mouse) Antibody, mAb (recombinant) (blocking)

  • E - Functional IL-33 (mouse) Antibody, mAb (recombinant) (blocking) ADP0008
    Binding of IL-33 (mouse) to ST2/IL-1RAcP is inhibited by IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1).
    Methods:IL-33 (mouse) was coated on an ELISA plate at 1ug/ml. IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1) or an unrelated mAb (recombinant) (Control) were added (starting at 40ug/ml with a twofold serial dilution) together with 100ul of supernatant of cells containing ST2 (human):Fc/IL-1RAcP (human):Fc. After incubation for 1h at RT, the binding was detected using an anti-Fc human antibody (HRP).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8BVZ5
Reactivity Mouse
Host Purified From HEK 293 Cell culture Supernatant.
Clonality Monoclonal
Isotype Mouse IgG2b
Gene Source Mouse
Endotoxin <0.01EU/ µg purified protein (LAL test; Lonza).
Application Note E,Functional Applicational, Inhibits the binding of mouse IL-33 to ST2/IL-1RAcP.
Clone Names Bondy-1-1
Calculated MW 29991 Da
Description anti-IL-33 (mouse), monoclonal antibody (recombinant) (Bondy-1-1) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the mouse lgG2b Fc domain.
Additional Information
Gene ID 77125
Other Names Interleukin-33; IL-1F11; NF-HEV
Target/Specificity Recognizes mouse IL-33.
Format Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Reconstitution & Storage Stable for at least 1 year after receipt when stored at -20°C.
PrecautionsFunctional IL-33 (mouse) Antibody, mAb (recombinant) (blocking) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Il33
Function Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.
Cellular Location Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Note=Associates with heterochromatin and mitotic chromosomes. Translocation from the nucleus occurs upon biomechanical strain, depends on an intact microtubule network, and is ATP-dependent (By similarity)
Research Areas
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Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released upon cell lysis. IL-33 binds to and signals through ST2 (IL-1R1) and its stimulation recruits MYD88, IRAK, IRAK4 and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38 (MAPK14) and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases and sepsis.

Anti-IL-33, mAb (recombinant) (blocking) (Bondy-1-1) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

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$ 350.00
Cat# ADP0008
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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