|Application ||IHC-P, IHC-F, FC, ICC|
|Host||Purified From Concentrated Hybridoma Tissue Culture Supernatant.|
|Endotoxin||<0.1EU/ µg purified protein (LAL test; Lonza).|
|Application Note||FC(1:100),IHC-F(1:200),IHC-P(1:200),ICC(1:200),Functional Applicational (Blocking), 250 µg / mouse (Optimal reagent to deplete neutrophils in vivo,)|
|Description||Negative control: Rat IgG2b Isotype Control"href="http://www.adipogen.com/ag-35b-0011/rat-igg2b-isotype-control.html"target="_self">AG-35B-0011|
|Target/Specificity||Recognizes mouse neutrophils (surface protein mouse Ly-6G).|
|Format||Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.|
|Reconstitution & Storage||Stable for at least 1 year after receipt when stored at -20°C.|
|Precautions||Functional Neutrophils (mouse) Antibody, mAb (blocking) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Neutrophils are key components of the inflammatory response and as such contribute to the killing of microorganisms. In addition, recent evidence suggests their involvement in the development of the immune response. The Nimp-R14 mAb displays features of an optimal reagent allowing the selective depletion of mouse neutrophils in vivo.
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