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Functional Rab6-GTP Antibody, mAb (recombinant)

  • ICC - Functional Rab6-GTP Antibody, mAb (recombinant) ADP0034
    Rab6-GTP is detected by immunocytochemistry using anti-Rab6-GTP, mAb (AA2).
    Method: HeLa cells are grown in standard culture conditions, fixed with paraformaldehyde (3%), permeablized in PBS+ BSA 0.2 % + Saponin 0.05 % and incubated with anti-Rab6-GTP, mAb (AA2) (1ug /ml) in PBS-BSA-Saponin. After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody in PBS-BSA-Saponin for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.
    Picture courtesy of Dr Moutel, Dr Franck Perez Lab, Curie Institute, Paris.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20340
Reactivity Human, Mouse, Drosophila
Host Purified From HEK 293 Cell culture Supernatant.
Clonality Monoclonal
Isotype Human IgG2λ
Gene Source Human
Application Note ICC(1:1'000)
Clone Names AA2
Calculated MW 23593 Da
Description anti-Rab6-GTP monoclonal antibody (recombinant) (AA2) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain.

anti-Rab6-GTP monoclonal antibody (recombinant) (AA2) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Additional Information
Gene ID 5870
Other Names Ras-related Protein Rab-6
Target/Specificity Recognizes human, mouse and Drosophila GTP-bound Rab6a and Rab6b and mutant Rab6Q72L. Does not detect Rab6•GDP.
Format Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Reconstitution & Storage Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
PrecautionsFunctional Rab6-GTP Antibody, mAb (recombinant) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB6A
Synonyms RAB6
Function Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity.
Cellular Location Golgi apparatus. Golgi apparatus membrane; Lipid- anchor
Tissue Location Ubiquitous..
Research Areas
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Rab6 is involved in protein transport. It is a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER).

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$ 325.00
Cat# ADP0034
(40 western blots)
Availability: 3-5 days
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