|Reactivity||Human, Mouse, Drosophila|
|Host||Purified From HEK 293 Cell culture Supernatant.|
|Calculated MW||23593 Da|
|Description||anti-Rab6-GTP monoclonal antibody (recombinant) (AA2) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain.|
anti-Rab6-GTP monoclonal antibody (recombinant) (AA2) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
|Other Names||Ras-related Protein Rab-6|
|Target/Specificity||Recognizes human, mouse and Drosophila GTP-bound Rab6a and Rab6b and mutant Rab6Q72L. Does not detect Rab6•GDP.|
|Format||Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.|
|Reconstitution & Storage||Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.|
|Precautions||Functional Rab6-GTP Antibody, mAb (recombinant) (ATTO488) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity. Involved in COPI-independent retrograde transport from the Golgi to the ER (PubMed:25962623).|
|Cellular Location||Golgi apparatus membrane; Lipid- anchor. Note=BICD2 facilitates its targeting to Golgi apparatus membrane Isoform 2: Golgi apparatus membrane; Lipid-anchor|
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Rab6 is involved in protein transport. It is a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER).
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