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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Functional Antibodies   >   Functional Myosin IIA (non-muscle) (heavy chain) Antibody, mAb (recombinant)   

Functional Myosin IIA (non-muscle) (heavy chain) Antibody, mAb (recombinant)

     
  • ICC - Functional Myosin IIA (non-muscle) (heavy chain) Antibody, mAb (recombinant) ADP0041
    Human Myosin IIA (non-muscle) (heavy chain) is detected by immunocytochemistry using anti-myosin IIA (non-muscle) (rec.) (SF9).
    Method:HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-Myosin IIA (non-muscle) (rec.) (SF9) (1mg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.
    Picture courtesy of Dr. Moutel, Dr. Franck Perez lab, Curie Institute, Paris.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC, E
Primary Accession Q9UKX2
Reactivity Human, Mouse, Rat, Drosophila
Host Purified From HEK 293 Cell culture Supernatant.
Clonality Monoclonal
Isotype Human IgG2λ
Gene Source Human
Application Note ,Electron Microscopy,E,ICC(1:1000),WB(1:1000)
Clone Names SF9
Calculated MW 223044 Da
Description anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain.
Additional Information
Gene ID 4620
Other Names Cellular Myosin Heavy Chain, Type A; Myosin Heavy Chain 9; Myosin Heavy Chain, Non-muscle IIa; Non-muscle Myosin Heavy Chain A
Target/Specificity Recognizes human, mouse, rat and drosophila myosin IIA (heavy chain).
Format Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Reconstitution & Storage Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
PrecautionsFunctional Myosin IIA (non-muscle) (heavy chain) Antibody, mAb (recombinant) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MYH2
Synonyms MYHSA2
Function Muscle contraction. Required for cytoskeleton organization (By similarity).
Cellular Location Cytoplasm, myofibril. Note=Thick filaments of the myofibrils
Research Areas
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Background

anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

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$ 300.00
Cat# ADP0041
Size:
Quantity:
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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