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Functional alpha-Tubulin Antibody, mAb (recombinant)

  • ICC - Functional alpha-Tubulin Antibody, mAb (recombinant) ADP0042
    Human alpha-Tubulin is detected by immunocytochemistry using anti-alpha-Tubulin, mAb (rec.) (F2C).
    Method:HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-alpha-Tubulin, mAb (rec.) (F2C) (1ug /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.
    Picture courtesy of Dr. Moutel, Dr. Franck Perez Lab, Curie Institute, Paris.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Human, Mouse, Bovine
Host Purified From HEK 293 Cell culture Supernatant.
Clonality Monoclonal
Isotype Human IgG2λ
Gene Source mouse
Application Note ICC(1:1'000),WB(1:1’000)
Clone Names F2C
Description anti-α-Tubulin, monoclonal antibody (recombinant) (F2C) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain.

anti-α-Tubulin, monoclonal antibody (recombinant) (F2C) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Additional Information
Target/Specificity Recognizes mouse, bovine and human α-tubulin.
Format Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Reconstitution & Storage Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
PrecautionsFunctional alpha-Tubulin Antibody, mAb (recombinant) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
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Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.

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$ 300.00
Cat# ADP0042
(40 western blots)
Availability: 3-5 days
Bulk Size
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