- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
4E-BP1 Antibody (T45)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AE1002a | 0.1 mg 400 ul | 7-10 days | $ 325.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
4E-BP1 Antibody (T45) - Product info | |
| Application | WB, IHC
|
| Primary Accession | Q13541 |
| Reactivity | Human, Mouse, Rat |
| Concentration | 1mg/ml |
| Isotype | Rabbit IgG |
| Calculated MW | 12580 Da |
4E-BP1 Antibody (T45) - Additional info | |
| Gene ID 1978 | |
| Other Names Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I; Eukaryotic translation initiation factor 4E-binding protein 1, eIF4E-binding protein 1; 4E-BP1 | |
| Target/Specificity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | |
| Dilution WB~~1:500~1:1000 IHC~~1:50-1:100 | |
| Format affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions 4E-BP1 Antibody (T45) is for research use only and not for use in diagnostic or therapeutic procedures. | |
4E-BP1 Antibody (T45) - Protein Information | |
| Name EIF4EBP1 | |
| Function Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways | |
4E-BP1 Antibody (T45) - Application data
-
Western blot analysis of extracts from MDA-MB-435 cells untreated or treated with EGF (200ng/ml, 5min), using 4E-BP1 Antibody (T45) (#AE1002a, Lane1 and 2) and Phospho-4E-BP1-T45 Antibody (#AE1002b, Lane 3 and 4).
-
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 Antibody (T45)(#AE1002a).
4E-BP1 Antibody (T45) - Related products
AP12627c: EIF4EBP1 Antibody (Center)
AP1981a: EIF4EBP1 Antibody (S100)
AP1981b: EIF4EBP1 Antibody (T36)
AP1981c: EIF4EBP1 Antibody (T69)
AP1981d: EIF4EBP1 Antibody(S111)
AP3353a: Phospho-EIF4EBP1-pS100 Antibody
AP3417a: Phospho-EIF4EBP1-pT36 Antibody
AP3473a: Phospho-EIF4EBP1-S111 Antibody
RI11733: EIF4EBP1 predesign siRNA
LY10995a: EIF4EBP1 Over-expression Lysate
BP1981a: EIF4EBP1 Antibody (S100) Blocking Peptide
BP1981b: EIF4EBP1 Antibody (T36) Blocking Peptide
BP1981c: EIF4EBP1 Antibody (T69) Blocking Peptide
BP3417a: Phospho-EIF4EBP1-pT36 Antibody Blocking Peptide
BP3473a: Phospho-EIF4EBP1-S111 Antibody Blocking Peptide
AJ1004d: 4E-BP1 Antibody Phospho (pT37)
AJ1004e: 4E-BP1 Antibody Phospho (pT70)
AJ1004f: 4E-BP1/2/3 Antibody Phospho (pT45)
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
REFERENCES
4E-BP1 is a key effector of the oncogenic activation of the AKT and ERK signaling pathways that integrates their function in tumors. She QB, et al. Cancer Cell, 2010 Jul 13. PMID 20609351. Use of genome-wide expression data to mine the Gray Zone of GWA studies leads to novel candidate obesity genes. Naukkarinen J, et al. PLoS Genet, 2010 Jun 3. PMID 20532202. Human cytomegalovirus UL69 protein facilitates translation by associating with the mRNA cap-binding complex and excluding 4EBP1. Aoyagi M, et al. Proc Natl Acad Sci U S A, 2010 Feb 9. PMID 20133758. The Parkinson's disease associated LRRK2 exhibits weaker in vitro phosphorylation of 4E-BP compared to autophosphorylation. Kumar A, et al. PLoS One, 2010 Jan 15. PMID 20090955. RhoE inhibits 4E-BP1 phosphorylation and eIF4E function impairing cap-dependent translation. Villalonga P, et al. J Biol Chem, 2009 Dec 18. PMID 19850923.
