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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ADD1 Antibody (S726)   

ADD1 Antibody (S726)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ADD1 Antibody (S726) AE1003a
    Western blot analysis of extract from HT-29 cells untreated or treated with Doxorubicin (1mM, 30min), using ADD1 Antibody (S726) (#AE1003a, Lane 1 and 2) and Phospho-ADD1-S726 Antibody (#AE1003b, Lane 3 and 4).
  • IHC - ADD1 Antibody (S726) AE1003a
    Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using ADD1 Antibody (S726) (#AE1003a).
  • IF - ADD1 Antibody (S726) AE1003a
    Immunofluorescence staining of methanol-fixed HeLa cells using ADD1 Antibody (S726) (#AE1003a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35611
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Concentration 1mg/ml
Isotype Rabbit IgG
Calculated MW 80955 Da
Additional Information
Gene ID 118
Other Names Alpha-adducin, Erythrocyte adducin subunit alpha, ADD1, ADDA
Target/Specificity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution WB~~1:500~1:1000
Format affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADD1 Antibody (S726) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADD1
Synonyms ADDA
Function Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Cellular Location Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side
Tissue Location Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit
Research Areas
Citations (0)

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Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.


Genetic risk factors for cerebral small-vessel disease in hypertensive patients from a genetically isolated population. Schuur M, et al. J Neurol Neurosurg Psychiatry, 2010 Jul 28. PMID 20667857.
Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.
Pharmacogenetic association of hypertension candidate genes with fasting glucose in the GenHAT Study. Irvin MR, et al. J Hypertens, 2010 Oct. PMID 20577119.
Population based allele frequencies of disease associated polymorphisms in the Personalized Medicine Research Project. Cross DS, et al. BMC Genet, 2010 Jun 17. PMID 20565774.
Independent predictive roles of eotaxin Ala23Thr, paraoxonase 2 Ser311Cys and beta-adrenergic receptor Trp64Arg polymorphisms on cardiac disease in Type 2 Diabetes--an 8-year prospective cohort analysis of 1297 patients. Wang Y, et al. Diabet Med, 2010 Apr. PMID 20536507.

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$ 325.00
Cat# AE1003a
(40 western blots)
Availability: 7-10 days
Bulk Size
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