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Phospho-Histone H3.1-S10 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Phospho-Histone H3.1-S10 Antibody AE1016d
    Western blot analysis of extract from HeLa cells using Histone H3.1 Antibody (S10) (#AE1016c, Lane 1 and 2) and Phospho-Histone H3.1-S10 Antibody (#AE1016d, Lane 3 and 4).
  • IHC - Phospho-Histone H3.1-S10 Antibody AE1016d
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-Histone H3.1-S10 Antibody (#AE1016d)(left) or the same antibody preincubated with blocking peptide(right).
  • IF - Phospho-Histone H3.1-S10 Antibody AE1016d
    Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear dot staining using Phospho-Histone H3.1-S10 Antibody (#AE1016d).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P68431
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Concentration 1mg/ml
Isotype Rabbit IgG
Calculated MW 15404 Da
Additional Information
Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968
Other Names Histone H31, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, H3FA
Target/Specificity The antibody was affinity-purified from rabbit antiserum using epitope-specific phosphopeptide column, and the antibody against non-phosphopeptide was removed using non-phosphopeptide column corresponding to the phosphorylation site.
Dilution WB~~1:500~1:1000
Format affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Histone H3.1-S10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms H3FA
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Research Areas
Citations (0)

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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.


PHF8 activates transcription of rRNA genes through H3K4me3 binding and H3K9me1/2 demethylation. Feng W, et al. Nat Struct Mol Biol, 2010 Apr. PMID 20208542.
Nucleosome formation activity of human somatic nuclear autoantigenic sperm protein (sNASP). Osakabe A, et al. J Biol Chem, 2010 Apr 16. PMID 20167597.
Structural biology of human H3K9 methyltransferases. Wu H, et al. PLoS One, 2010 Jan 11. PMID 20084102.
Molecular functions of the histone acetyltransferase chaperone complex Rtt109-Vps75. Berndsen CE, et al. Nat Struct Mol Biol, 2008 Sep. PMID 19172748.
Np95 is a histone-binding protein endowed with ubiquitin ligase activity. Citterio E, et al. Mol Cell Biol, 2004 Mar. PMID 14993289.

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$ 325.00
Cat# AE1016d
(40 western blots)
Availability: 7-10 days
Bulk Size
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