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Goat Anti-58KGolgi protein(Internal)/FTCD Antibody

Peptide-affinity purified goat antibody

     
  • WB - Goat Anti-58KGolgi protein(Internal)/FTCD Antibody AF1003b
    AF1003b (0.1 µg/ml) staining of Human (A), (0.03ug/ml) of Mouse (B) and (0.3ug/ml) of Pig (C) Liver lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
    detail
  • IHC - Goat Anti-58KGolgi protein(Internal)/FTCD Antibody AF1003b
    AF1003b (3.75 µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • IF - Goat Anti-58KGolgi protein(Internal)/FTCD Antibody AF1003b
    AF1003b Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic and plasma membrane stain
    detail
  • IF - Goat Anti-58KGolgi protein(Internal)/FTCD Antibody AF1003b
    AF1003b Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane staining. The nuclear stain is DA
    detail
  • FC - Goat Anti-58KGolgi protein(Internal)/FTCD Antibody AF1003b
    AF1003b Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession O95954
Other Accession NP_996848, 10841
Reactivity Human
Predicted Mouse, Rat, Pig
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 58927 Da
Additional Information
Gene ID 10841
Other Names Formimidoyltransferase-cyclodeaminase, Formiminotransferase-cyclodeaminase, FTCD, LCHC1, Glutamate formimidoyltransferase, 2.1.2.5, Glutamate formiminotransferase, Glutamate formyltransferase, Formimidoyltetrahydrofolate cyclodeaminase, 4.3.1.4, Formiminotetrahydrofolate cyclodeaminase, FTCD
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-58KGolgi protein(Internal)/FTCD Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FTCD
Function Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
Cellular Location Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9YH58}. Golgi apparatus {ECO:0000250|UniProtKB:Q9YH58}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Note=More abundantly located around the mother centriole
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.

References

Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia. Jugessur A, et al. PLoS One, 2010 Jul 9. PMID 20634891.
Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.
Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. Talmud PJ, et al. Am J Hum Genet, 2009 Nov. PMID 19913121.
Transcriptomic and genetic studies identify IL-33 as a candidate gene for Alzheimer's disease. Chapuis J, et al. Mol Psychiatry, 2009 Nov. PMID 19204726.
An association study of 45 folate-related genes in spina bifida: Involvement of cubilin (CUBN) and tRNA aspartic acid methyltransferase 1 (TRDMT1). Franke B, et al. Birth Defects Res A Clin Mol Teratol, 2009 Mar. PMID 19161160.

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$ 341.00
Cat# AF1003b
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