|Application ||WB, E|
|Other Accession||NP_009133, 11216, 56697 (mouse), 360540 (rat)|
|Predicted||Human, Mouse, Dog, Cow|
|Calculated MW||73818 Da|
|Other Names||A-kinase anchor protein 10, mitochondrial, AKAP-10, Dual specificity A kinase-anchoring protein 2, D-AKAP-2, Protein kinase A-anchoring protein 10, PRKA10, AKAP10|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-AKAP10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA. It cannot be excluded too that it may facilitate PKA as well as G protein signal transduction, by acting as an adapter for assembling multiprotein complexes. With its RGS domain, it could lead to the interaction to G-alpha proteins, providing a link between the signaling machinery and the downstream kinase (By similarity).|
|Cellular Location||Mitochondrion. Membrane. Cytoplasm. Note=Predominantly mitochondrial but also membrane associated and cytoplasmic|
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Provided below are standard protocols that you may find useful for product applications.
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA; therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction.
A Large-scale genetic association study of esophageal adenocarcinoma risk. Liu CY, et al. Carcinogenesis, 2010 Jul. PMID 20453000.
Structure of D-AKAP2:PKA RI complex: insights into AKAP specificity and selectivity. Sarma GN, et al. Structure, 2010 Feb 10. PMID 20159461.
Association of the A1936G (rs203462) of A-kinase anchoring protein 10 polymorphisms with QT interval prolongation during kidney transplantation. Zukowski M, et al. Transplant Proc, 2009 Oct. PMID 19857670.
D-AKAP2 interacts with Rab4 and Rab11 through its RGS domains and regulates transferrin receptor recycling. Eggers CT, et al. J Biol Chem, 2009 Nov 20. PMID 19797056.
Association of genetic variants with chronic kidney disease in individuals with different lipid profiles. Yoshida T, et al. Int J Mol Med, 2009 Aug. PMID 19578796.
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