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Goat Anti-AKAP8 / AKAP95 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-AKAP8 / AKAP95 Antibody AF1044a
    AF1044a staining (1 µg/ml) of Human Liver lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43823
Other Accession NP_005849, 10270, 56399 (mouse), 116633 (rat)
Reactivity Human
Predicted Mouse, Rat, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 76108 Da
Additional Information
Gene ID 10270
Other Names A-kinase anchor protein 8, AKAP-8, A-kinase anchor protein 95 kDa, AKAP 95, AKAP8, AKAP95
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-AKAP8 / AKAP95 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AKAP8
Synonyms AKAP95
Function Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).
Cellular Location Nucleus matrix. Note=Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis
Tissue Location Highly expressed in heart, liver, skeletal muscle, kidney and pancreas
Research Areas
Citations (0)

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The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.


Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Olsen JV, et al. Cell, 2006 Nov 3. PMID 17081983.
A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Beausoleil SA, et al. Nat Biotechnol, 2006 Oct. PMID 16964243.
A-kinase-anchoring protein 95 functions as a potential carrier for the nuclear translocation of active caspase 3 through an enzyme-substrate-like association. Kamada S, et al. Mol Cell Biol, 2005 Nov. PMID 16227597.
Large-scale characterization of HeLa cell nuclear phosphoproteins. Beausoleil SA, et al. Proc Natl Acad Sci U S A, 2004 Aug 17. PMID 15302935.

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$ 325.00
Cat# AF1044a
(40 western blots)
Availability: 7-10 days
Bulk Size
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