|Application ||WB, IHC, E|
|Other Accession||NP_671714, 10142|
|Calculated MW||453667 Da|
|Other Names||A-kinase anchor protein 9, AKAP-9, A-kinase anchor protein 350 kDa, AKAP 350, hgAKAP 350, A-kinase anchor protein 450 kDa, AKAP 450, AKAP 120-like protein, Centrosome- and Golgi-localized PKN-associated protein, CG-NAP, Protein hyperion, Protein kinase A-anchoring protein 9, PRKA9, Protein yotiao, AKAP9, AKAP350, AKAP450, KIAA0803|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-AKAP9 / AKAP450 / CG-NAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||AKAP350, AKAP450, KIAA0803|
|Function||Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. Required to maintain the integrity of the Golgi apparatus (PubMed:10202149, PubMed:15047863). Recruited to the Golgi apparatus by GM130/GOLGA2 and is required for microtubule nucleation at the cis-side of the Golgi apparatusGM130/GOLGA2.|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Golgi apparatus. Note=Cytoplasmic in parietal cells|
|Tissue Location||Widely expressed (PubMed:10202149). Isoform 4: Highly expressed in skeletal muscle and in pancreas (PubMed:9482789).|
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Provided below are standard protocols that you may find useful for product applications.
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternate splicing of this gene results in at least two isoforms that localize to the centrosome and the Golgi apparatus, and interact with numerous signaling proteins from multiple signal transduction pathways. These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, protein phosphatase 2a, protein kinase C-epsilon and phosphodiesterase 4D3.
A Large-scale genetic association study of esophageal adenocarcinoma risk. Liu CY, et al. Carcinogenesis, 2010 Jul. PMID 20453000.
Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.
Integrin and CD3/TCR activation are regulated by the scaffold protein AKAP450. Robles-Valero J, et al. Blood, 2010 May 27. PMID 20231423.
International Lung Cancer Consortium: coordinated association study of 10 potential lung cancer susceptibility variants. Truong T, et al. Carcinogenesis, 2010 Apr. PMID 20106900.
Microtubule nucleation at the cis-side of the Golgi apparatus requires AKAP450 and GM130. Rivero S, et al. EMBO J, 2009 Apr 22. PMID 19242490.
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