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Goat Anti-Amphiphysin / AMPH Antibody

Peptide-affinity purified goat antibody

     
  • IHC - Goat Anti-Amphiphysin / AMPH Antibody AF1060a
    AF1060a (2.5 µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • WB - Goat Anti-Amphiphysin / AMPH Antibody AF1060a
    AF1060a (0.5 µg/ml) staining of mouse brain lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P49418
Other Accession NP_647477, 273, 218038 (mouse)
Reactivity Human, Mouse
Predicted Rat, Dog
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 76257 Da
Additional Information
Gene ID 273
Other Names Amphiphysin, AMPH, AMPH1
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-Amphiphysin / AMPH Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AMPH
Synonyms AMPH1
Function May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
Cellular Location Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasm, cytoskeleton
Tissue Location Neurons, certain endocrine cell types and spermatocytes
Research Areas
Citations (0)
citation

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Background

This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined.

References

Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.
Applying chemometrics approaches to model and predict the binding affinities between the human amphiphysin SH3 domain and its peptide ligands. Liu L, et al. Protein Pept Lett, 2010 Feb. PMID 20214647.
Toward quantitative characterization of the binding profile between the human amphiphysin-1 SH3 domain and its peptide ligands. He P, et al. Amino Acids, 2010 Apr. PMID 19669081.
Modeling and prediction of binding affinities between the human amphiphysin SH3 domain and its peptide ligands using genetic algorithm-Gaussian processes. Zhou P, et al. Biopolymers, 2008. PMID 18814309.
Characterization of domain-peptide interaction interface: a case study on the amphiphysin-1 SH3 domain. Hou T, et al. J Mol Biol, 2008 Feb 29. PMID 18206907.

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$ 341.00
Cat# AF1060a
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