|Application ||WB, E|
|Other Accession||NP_057106, 51107|
|Predicted||Mouse, Rat, Pig, Dog, Cow|
|Calculated MW||28996 Da|
|Other Names||Gamma-secretase subunit APH-1A, APH-1a, Aph-1alpha, Presenilin-stabilization factor, APH1A, PSF|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-APH1A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Non-catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed:12297508, PubMed:12522139, PubMed:12763021, PubMed:12679784, PubMed:25043039, PubMed:26280335). Required for normal gamma- secretase assembly (PubMed:12522139, PubMed:12471034, PubMed:12763021, PubMed:19369254). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (Probable).|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi-pass membrane protein. Note=Predominantly located in the endoplasmic reticulum and in the cis-Golgi|
|Tissue Location||Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level.|
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Provided below are standard protocols that you may find useful for product applications.
APH1 is a multipass transmembrane protein that interacts with presenilin (see PSEN1; MIM 104311) and nicastrin (APH2; MIM 605254) as a functional component of the gamma-secretase complex. The gamma-secretase complex is required for the intramembrane proteolysis of a number of membrane proteins, including the amyloid-beta precursor protein (APP; MIM 104760) and Notch (MIM 190198).
Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.
Human CRB2 inhibits gamma-secretase cleavage of amyloid precursor protein by binding to the presenilin complex. Mitsuishi Y, et al. J Biol Chem, 2010 May 14. PMID 20299451.
Aph-1 associates directly with full-length and C-terminal fragments of gamma-secretase substrates. Chen AC, et al. J Biol Chem, 2010 Apr 9. PMID 20145246.
APH1 polar transmembrane residues regulate the assembly and activity of presenilin complexes. Pardossi-Piquard R, et al. J Biol Chem, 2009 Jun 12. PMID 19369254.
Association between promoter polymorphisms in anterior pharynx-defective-1a and sporadic Alzheimer's disease in the North Chinese Han population. Wang Y, et al. Neurosci Lett, 2009 May 15. PMID 19368855.
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