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Goat Anti-ARF1, 2, 3, 4 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-ARF1, 2, 3, 4 Antibody AF1094a
    AF1094a (1 µg/ml) staining of HepG2 cell lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P84077
Other Accession NP_001019399, 375, 376, 377, 378, 11840 (mouse), 64310 (rat)
Reactivity Human
Predicted Mouse, Rat, Dog
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 20697 Da
Additional Information
Gene ID 375
Other Names ADP-ribosylation factor 1, ARF1
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-ARF1, 2, 3, 4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARF1
Function GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).
Cellular Location Golgi apparatus. Cytoplasm, perinuclear region. Cell junction, synapse, synaptosome. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density
Research Areas
Citations (0)

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ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.


Regulation of mTORC1 by the Rab and Arf GTPases. Li L, et al. J Biol Chem, 2010 Jun 25. PMID 20457610.
Protein complexes containing CYFIP/Sra/PIR121 coordinate Arf1 and Rac1 signalling during clathrin-AP-1-coated carrier biogenesis at the TGN. Anitei M, et al. Nat Cell Biol, 2010 Apr. PMID 20228810.
Modifications to the C-terminus of Arf1 alter cell functions and protein interactions. Jian X, et al. Traffic, 2010 Jun. PMID 20214751.
Role of the GTPase Rab1b in ebolavirus particle formation. Yamayoshi S, et al. J Virol, 2010 May. PMID 20164217.
Role of the second cysteine-rich domain and Pro275 in protein kinase D2 interaction with ADP-ribosylation factor 1, trans-Golgi network recruitment, and protein transport. Pusapati GV, et al. Mol Biol Cell, 2010 Mar. PMID 20089835.

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$ 325.00
Cat# AF1094a
(40 western blots)
Availability: 7-10 days
Bulk Size
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