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Goat Anti-ATF7 Antibody

Peptide-affinity purified goat antibody

  • IHC - Goat Anti-ATF7 Antibody AF1124a
    AF1124a (3.8 µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • WB - Goat Anti-ATF7 Antibody AF1124a
    AF1124a staining (0.5 µg/ml) of HeLa lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P17544
Other Accession NP_001123532, 11016
Reactivity Human
Predicted Rat, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 52967 Da
Additional Information
Gene ID 11016
Other Names Cyclic AMP-dependent transcription factor ATF-7, cAMP-dependent transcription factor ATF-7, Activating transcription factor 7, Transcription factor ATF-A, ATF7, ATFA
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-ATF7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATF7
Synonyms ATFA
Function Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]- 3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family. Isoform 5/ATF-4 acts as a negative regulator, inhibiting both ATF2 and ATF7 transcriptional activities. It may exert these effects by sequestrating in the cytoplasm the Thr-53 phosphorylating kinase, preventing activation.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00978, ECO:0000269|PubMed:17264123}. Nucleus, nucleoplasm. Note=Mainly nucleoplasmic. Restricted distribution to the perinuculear region The sumoylated form locates to the nuclear periphery
Tissue Location Expressed in heart, lung and skeletal muscle. Isoform 4 is expressed in various tissues including heart, brain, placenta, lung and skeletal muscle. Highest levels in skeletal muscle. Lowest in lung and placenta.
Research Areas
Citations (0)

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A genome-wide association study in 19 633 Japanese subjects identified LHX3-QSOX2 and IGF1 as adult height loci. Okada Y, et al. Hum Mol Genet, 2010 Jun 1. PMID 20189936.
p38beta2-mediated phosphorylation and sumoylation of ATF7 are mutually exclusive. Camuzeaux B, et al. J Mol Biol, 2008 Dec 26. PMID 18950637.
IRF2-binding protein-1 is a JDP2 ubiquitin ligase and an inhibitor of ATF2-dependent transcription. Kimura M. FEBS Lett, 2008 Aug 20. PMID 18671972.
Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.
Sumoylation delays the ATF7 transcription factor subcellular localization and inhibits its transcriptional activity. Hamard PJ, et al. Nucleic Acids Res, 2007. PMID 17264123.

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$ 325.00
Cat# AF1124a
(40 western blots)
Availability: 7-10 days
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