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Goat Anti-BARK1 / GRK2 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-BARK1 / GRK2 Antibody AF1142a
    AF1142a (0.3 µg/ml) staining of Daudi cell lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P25098
Other Accession NP_001610, 156, 110355 (mouse)
Reactivity Human
Predicted Mouse, Rat, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 79574 Da
Additional Information
Gene ID 156
Other Names Beta-adrenergic receptor kinase 1, Beta-ARK-1,, G-protein coupled receptor kinase 2, ADRBK1, BARK, BARK1, GRK2
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-BARK1 / GRK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms BARK, BARK1, GRK2
Function Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:P21146}. Cell membrane {ECO:0000250|UniProtKB:P21146}
Tissue Location Expressed in peripheral blood leukocytes.
Research Areas
Citations (0)

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The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.


Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.
Association study of 182 candidate genes in anorexia nervosa. Pinheiro AP, et al. Am J Med Genet B Neuropsychiatr Genet, 2010 Jul. PMID 20468064.
Internalization of the human nicotinic acid receptor GPR109A is regulated by G(i), GRK2, and arrestin3. Li G, et al. J Biol Chem, 2010 Jul 16. PMID 20460384.
Dynamic changes in lymphocyte GRK2 levels in cardiac transplant patients: a biomarker for left ventricular function. Bonita RE, et al. Clin Transl Sci, 2010 Feb. PMID 20443948.
Enhanced GRK2 expression and desensitization of betaAR vasodilatation in hypertensive patients. Izzo R, et al. Clin Transl Sci, 2008 Dec. PMID 20443852.

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$ 325.00
Cat# AF1142a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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