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Goat Anti-BRF2 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-BRF2 Antibody AF1167a
    AF1167a staining (1.5 µg/ml) of Hela lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9HAW0
Other Accession NP_060780, 55290
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 46533 Da
Additional Information
Gene ID 55290
Other Names Transcription factor IIIB 50 kDa subunit, TFIIIB50, hTFIIIB50, B-related factor 2, BRF-2, hBRFU, BRF2, BRFU
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-BRF2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BRF2
Synonyms BRFU
Function General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites.
Cellular Location Nucleus.
Research Areas
Citations (0)

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This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein.


Differential expression of the TFIIIB subunits Brf1 and Brf2 in cancer cells. Cabarcas S, et al. BMC Mol Biol, 2008 Aug 12. PMID 18700021.
Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.
Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Kimura K, et al. Genome Res, 2006 Jan. PMID 16344560.
Structure-function analysis of the human TFIIB-related factor II protein reveals an essential role for the C-terminal domain in RNA polymerase III transcription. Saxena A, et al. Mol Cell Biol, 2005 Nov. PMID 16227591.
Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039.

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$ 325.00
Cat# AF1167a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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