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Goat Anti-Calponin 2 / CNN2 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-Calponin 2 / CNN2 Antibody AF1180a
    AF1180a (0.03 µg/ml) staining of HepG2 lysates (35 µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99439
Other Accession NP_958434, 1265
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 33697 Da
Additional Information
Gene ID 1265
Other Names Calponin-2, Calponin H2, smooth muscle, Neutral calponin, CNN2
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-Calponin 2 / CNN2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CNN2
Function Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Tissue Location Heart and smooth muscle.
Research Areas
Citations (0)

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The protein encoded by this gene, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. The encoded protein could play a role in smooth muscle contraction and cell adhesion. Two transcript variants encoding different isoforms have been found for this gene.


h2-Calponin is regulated by mechanical tension and modifies the function of actin cytoskeleton. Hossain MM, et al. J Biol Chem, 2005 Dec 23. PMID 16236705.
Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039.
Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. Gevaert K, et al. Nat Biotechnol, 2003 May. PMID 12665801.
Developmentally regulated expression of calponin isoforms and the effect of h2-calponin on cell proliferation. Hossain MM, et al. Am J Physiol Cell Physiol, 2003 Jan. PMID 12388067.
The DNA sequence of human chromosome 21. Hattori M, et al. Nature, 2000 May 18. PMID 10830953.

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$ 325.00
Cat# AF1180a
(40 western blots)
Availability: 7-10 days
Bulk Size
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