- CITATIONS: 1
|Application ||WB, E|
|Other Accession||NP_071445, 64127, 257632 (mouse), 291912 (rat)|
|Calculated MW||115283 Da|
|Other Names||Nucleotide-binding oligomerization domain-containing protein 2, Caspase recruitment domain-containing protein 15, Inflammatory bowel disease protein 1, NOD2, CARD15, IBD1|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-CARD15 / NOD2 (Internal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in gastrointestinal immunity. Upon stimulation by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, binds the proximal adapter receptor-interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3, INAVA and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling. This in turn leads to the transcriptional activation of hundreds of genes involved in immune response. Required for MDP-induced NLRP1-dependent CASP1 activation and IL1B release in macrophages (PubMed:18511561). Component of an autophagy-mediated antibacterial pathway together with ATG16L1 (PubMed:20637199). Plays also a role in sensing single-stranded RNA (ssRNA) from viruses. Interacts with mitochondrial antiviral signaling/MAVS, leading to activation of interferon regulatory factor-3/IRF3 and expression of type I interferon (PubMed:19701189).|
|Cellular Location||Cytoplasm Membrane. Mitochondrion. Basolateral cell membrane|
|Tissue Location||Expressed in intestinal mucosa, mainly in Paneth cells and, at lower extent, in the glandular epithelium|
Provided below are standard protocols that you may find useful for product applications.
This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohn disease and Blau syndrome.
NOD2 mutations predict the risk for surgery in pediatric-onset Crohn's disease. Lacher M, et al. J Pediatr Surg, 2010 Aug. PMID 20713205.
CARD15 mutations and colorectal cancer in a South European country. Freire P, et al. Int J Colorectal Dis, 2010 Oct. PMID 20676658.
A genetic association study of maternal and fetal candidate genes that predispose to preterm prelabor rupture of membranes (PROM). Romero R, et al. Am J Obstet Gynecol, 2010 Jul 29. PMID 20673868.
Toll-like receptor (TLR) and nucleosome-binding oligomerization domain (NOD) gene polymorphisms and endometrial cancer risk. Ashton KA, et al. BMC Cancer, 2010 Jul 21. PMID 20646321.
NOD2 polymorphisms in clinical phenotypes of common variable immunodeficiency disorders. Packwood K, et al. Clin Exp Immunol, 2010 Jul 14. PMID 20646002.
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