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Goat Anti-CBX5 / HP1-Alpha Antibody

Peptide-affinity purified goat antibody

  • IHC - Goat Anti-CBX5 / HP1-Alpha Antibody AF1205a
    AF1205a (2.5 µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • WB - Goat Anti-CBX5 / HP1-Alpha Antibody AF1205a
    AF1205a (0.03 µg/ml) staining of Hela lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P45973
Other Accession NP_001120794, 23468
Reactivity Human
Predicted Mouse, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 22225 Da
Additional Information
Gene ID 23468
Other Names Chromobox protein homolog 5, Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha, CBX5, HP1A
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-CBX5 / HP1-Alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CBX5
Synonyms HP1A
Function Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Cellular Location Nucleus. Chromosome. Chromosome, centromere. Note=Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase
Research Areas
Citations (0)

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This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.


Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation. Nozawa RS, et al. Nat Cell Biol, 2010 Jul. PMID 20562864.
Lamin A rod domain mutants target heterochromatin protein 1alpha and beta for proteasomal degradation by activation of F-box protein, FBXW10. Chaturvedi P, et al. PLoS One, 2010 May 13. PMID 20498703.
Inner centromere formation requires hMis14, a trident kinetochore protein that specifically recruits HP1 to human chromosomes. Kiyomitsu T, et al. J Cell Biol, 2010 Mar 22. PMID 20231385.
Protein complex of Drosophila ATRX/XNP and HP1a is required for the formation of pericentric beta-heterochromatin in vivo. Emelyanov AV, et al. J Biol Chem, 2010 May 14. PMID 20154359.
ATRX interacts with H3.3 in maintaining telomere structural integrity in pluripotent embryonic stem cells. Wong LH, et al. Genome Res, 2010 Mar. PMID 20110566.

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$ 325.00
Cat# AF1205a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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