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Goat Anti-CD2BP2 (N Terminus) Antibody

Peptide-affinity purified goat antibody

     
  • WB - Goat Anti-CD2BP2 (N Terminus) Antibody AF1216a
    AF1216a (0.3 µg/ml) staining of Human Thymus lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • IHC - Goat Anti-CD2BP2 (N Terminus) Antibody AF1216a
    AF1216a (5 µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession O95400
Other Accession NP_006101, 10421
Reactivity Human
Predicted Mouse, Rat, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Additional Information
Other Names CD2 antigen cytoplasmic tail-binding protein 2, CD2 cytoplasmic domain-binding protein 2, CD2 tail-binding protein 2, U5 snRNP 52K protein, U5-52K, CD2BP2, KIAA1178
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-CD2BP2 (N Terminus) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD2BP2
Synonyms KIAA1178
Function Involved in pre-mRNA splicing as component of the U5 snRNP complex that is involved in spliceosome assembly.
Cellular Location Cytoplasm. Nucleus. Note=Predominantly nuclear EMBL; AF104222; AAC84141.1; -; mRNA EMBL; AK315708; BAG38069.1; -; mRNA EMBL; CH471192; EAW52260.1; -; Genomic_DNA EMBL; BC000495; AAH00495.1; -; mRNA EMBL; BC001947; AAH01947.1; -; mRNA EMBL; AB033004; BAA86492.1; -; mRNA CCDS; CCDS10675.1; - RefSeq; NP_001230575.1; NM_001243646.1 RefSeq; NP_006101.1; NM_006110.2 UniGene; Hs.202677; - PDB; 1GYF; NMR; -; A=280-341 PDB; 1L2Z; NMR; -; A=280-341 PDB; 1SYX; X-ray; 2.34 A; B/D/F=262-341 PDB; 4BWS; X-ray; 2.50 A; C/F=280-341 PDBsum; 1GYF; - PDBsum; 1L2Z; - PDBsum; 1SYX; - PDBsum; 4BWS; - ProteinModelPortal; O95400; - SMR; O95400; - BioGrid; 115690; 143 CORUM; O95400; - ELM; O95400; - IntAct; O95400; 67 MINT; O95400; - STRING; 9606.ENSP00000304903; - iPTMnet; O95400; - PhosphoSitePlus; O95400; - BioMuta; CD2BP2; - EPD; O95400; - MaxQB; O95400; - PaxDb; O95400; - PeptideAtlas; O95400; - PRIDE; O95400; - ProteomicsDB; 50853; - DNASU; 10421; - Ensembl; ENST00000305596; ENSP00000304903; ENSG00000169217 Ensembl; ENST00000569466; ENSP00000456935; ENSG00000169217 GeneID; 10421; - KEGG; hsa:10421; - UCSC; uc002dxr.4; human CTD; 10421; - DisGeNET; 10421; - EuPathDB; HostDB:ENSG00000169217.8; - GeneCards; CD2BP2; - HGNC; HGNC:1656; CD2BP2 HPA; HPA041508; - HPA; HPA061309; - MIM; 604470; gene neXtProt; NX_O95400; - OpenTargets; ENSG00000169217; - PharmGKB; PA26209; - eggNOG; KOG2950; Eukaryota eggNOG; ENOG4111KF5; LUCA GeneTree; ENSGT00390000012483; - HOGENOM; HOG000007061; - HOVERGEN; HBG056920; - InParanoid; O95400; - KO; K13099; - OMA; TGNMDIY; - OrthoDB; EOG091G0MVR; - PhylomeDB; O95400; - TreeFam; TF313042; - Reactome; R-HSA-72163; mRNA Splicing - Major Pathway ChiTaRS; CD2BP2; human EvolutionaryTrace; O95400; - GeneWiki; CD2BP2; - GenomeRNAi; 10421; - PRO; PR:O95400; - Proteomes; UP000005640; Chromosome 16 Bgee; ENSG00000169217; - CleanEx; HS_CD2BP2; - ExpressionAtlas; O95400; baseline and differential Genevisible; O95400; HS GO; GO:0005737; C:cytoplasm; TAS:ProtInc GO; GO:0005829; C:cytosol; IDA:HPA GO; GO:0001650; C:fibrillar center; IDA:HPA GO; GO:0016607; C:nuclear speck; IDA:HPA GO; GO:0005654; C:nucleoplasm; IDA:BHF-UCL GO; GO:0005634; C:nucleus; IDA:BHF-UCL GO; GO:0005682; C:U5 snRNP; IDA:BHF-UCL GO; GO:0043021; F:ribonucleoprotein complex binding; IDA:BHF-UCL GO; GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome GO; GO:0010923; P:negative regulation of phosphatase activity; IDA:UniProtKB GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; NAS:BHF-UCL CDD; cd00072; GYF; 1 Gene3D; 3.30.1490.40; -; 1 InterPro; IPR003169; GYF InterPro; IPR035445; GYF-like_dom_sf Pfam; PF02213; GYF; 1 SMART; SM00444; GYF; 1 SUPFAM; SSF55277; SSF55277; 1 PROSITE; PS50829; GYF; 1 1: Evidence at protein level; 3D-structure; Acetylation; Complete proteome; Cytoplasm; Isopeptide bond; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation CHAIN 1 341 CD2 antigen cytoplasmic tail-binding protein 2 /FTId=PRO_0000089437 DOMAIN 280 338 GYF. {ECO:0000255|PROSITE- ProRule:PRU00101} MOD_RES 44 44 N6-acetyllysine {ECO:0000250|UniProtKB:Q9CWK3} MOD_RES 46 46 Phosphoserine MOD_RES 49 49 Phosphoserine MOD_RES 118 118 Phosphoserine MOD_RES 194 194 Phosphoserine MOD_RES 195 195 Phosphoserine CROSSLNK 26 26 Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) VARIANT 231 231 G -> D (in dbSNP:rs13330462) /FTId=VAR_050772 VARIANT 262 262 T -> I (in dbSNP:rs34391305) /FTId=VAR_050773 STRAND 282 293 {ECO:0000244|PDB:1SYX} STRAND 296 300 {ECO:0000244|PDB:1SYX} HELIX 301 309 {ECO:0000244|PDB:1SYX} STRAND 318 324 {ECO:0000244|PDB:1SYX} HELIX 332 334 {ECO:0000244|PDB:1SYX} HELIX 337 340 {ECO:0000244|PDB:1SYX} SEQUENCE 341 AA; 37646 MW; 8E9A7EE0C40474D5 CRC64; MPKRKVTFQG VGDEEDEDEI IVPKKKLVDP VAGSGGPGSR FKGKHSLDSD EEEDDDDGGS SKYDILASED VEGQEAATLP SEGGVRITPF NLQEEMEEGH FDADGNYFLN RDAQIRDSWL DNIDWVKIRE RPPGQRQASD SEEEDSLGQT SMSAQALLEG LLELLLPRET VAGALRRLGA RGGGKGRKGP GQPSSPQRLD RLSGLADQMV ARGNLGVYQE TRERLAMRLK GLGCQTLGPH NPTPPPSLDM FAEELAEEEL ETPTPTQRGE AESRGDGLVD VMWEYKWENT GDAELYGPFT SAQMQTWVSE GYFPDGVYCR KLDPPGGQFY NSKRIDFDLY T
Research Areas
Citations (0)

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Background

This gene encodes a bi-functional protein. In the cytoplasm, the encoded protein binds the cytoplasmic tail of human surface antigen CD2 via its C-terminal GYF domain, and regulate CD2-triggered T lymphocyte activation. In the nucleus, this protein is a component of the U5 small nuclear ribonucleoprotein complex and is involved in RNA splicing. A pseudogene has been identified on chromosome 7. Alternative splicing results in multiple transcript variants but their biological validity has not been determined.

References

Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.
Investigating the functional role of CD2BP2 in T cells. Heinze M, et al. Int Immunol, 2007 Nov. PMID 17906334.
Structural basis for the bifunctionality of the U5 snRNP 52K protein (CD2BP2). Nielsen TK, et al. J Mol Biol, 2007 Jun 15. PMID 17467737.
Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Olsen JV, et al. Cell, 2006 Nov 3. PMID 17081983.

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$ 335.00
Cat# AF1216a
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