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Goat Anti-CDT1 / Dup Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-CDT1 / Dup Antibody AF1223a
    AF1223a (0.05 µg/ml) staining of Human Ovary lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H211
Other Accession NP_112190, 81620
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 60390 Da
Additional Information
Gene ID 81620
Other Names DNA replication factor Cdt1, Double parked homolog, DUP, CDT1 {ECO:0000312|EMBL:AAG45181.1}
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-CDT1 / Dup Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDT1 {ECO:0000312|EMBL:AAG45181.1}
Function Cooperates with CDC6 to promote the loading of the mini- chromosome maintenance complex onto chromatin to form the pre- replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.
Cellular Location Nucleus.
Research Areas
Citations (0)

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Quaternary structure of the human Cdt1-Geminin complex regulates DNA replication licensing. De Marco V, et al. Proc Natl Acad Sci U S A, 2009 Nov 24. PMID 19906994.
Acetylation/deacetylation modulates the stability of DNA replication licensing factor Cdt1. Glozak MA, et al. J Biol Chem, 2009 Apr 24. PMID 19276081.
Human CDT1 associates with CDC7 and recruits CDC45 to chromatin during S phase. Ballabeni A, et al. J Biol Chem, 2009 Jan 30. PMID 19054765.
HBO1 histone acetylase is a coactivator of the replication licensing factor Cdt1. Miotto B, et al. Genes Dev, 2008 Oct 1. PMID 18832067.
MCM9 binds Cdt1 and is required for the assembly of prereplication complexes. Lutzmann M, et al. Mol Cell, 2008 Jul 25. PMID 18657502.

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$ 325.00
Cat# AF1223a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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