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Goat Anti-CDT1 / Dup Antibody
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9H211 |
| Other Accession | NP_112190, 81620 |
| Reactivity | Human |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 100ug/200ul |
| Isotype | IgG |
| Calculated MW | 60390 Da |
| Gene ID | 81620 |
|---|---|
| Other Names | DNA replication factor Cdt1, Double parked homolog, DUP, CDT1 {ECO:0000312|EMBL:AAG45181.1} |
| Format | 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Goat Anti-CDT1 / Dup Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CDT1 {ECO:0000312|EMBL:AAG45181.1} |
|---|---|
| Function | Required for both DNA replication and mitosis (PubMed:11125146, PubMed:22581055, PubMed:21856198, PubMed:14993212, PubMed:26842564). DNA replication licensing factor, required for pre- replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC)(PubMed:14672932). Required also for mitosis by promoting stable kinetochore-microtubule attachments (PubMed:22581055). Potential oncogene (By similarity). |
| Cellular Location | Nucleus. Chromosome, centromere, kinetochore. Note=Transiently localizes to kinetochores during prometaphase and metaphase |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
References
Quaternary structure of the human Cdt1-Geminin complex regulates DNA replication licensing. De Marco V, et al. Proc Natl Acad Sci U S A, 2009 Nov 24. PMID 19906994.
Acetylation/deacetylation modulates the stability of DNA replication licensing factor Cdt1. Glozak MA, et al. J Biol Chem, 2009 Apr 24. PMID 19276081.
Human CDT1 associates with CDC7 and recruits CDC45 to chromatin during S phase. Ballabeni A, et al. J Biol Chem, 2009 Jan 30. PMID 19054765.
HBO1 histone acetylase is a coactivator of the replication licensing factor Cdt1. Miotto B, et al. Genes Dev, 2008 Oct 1. PMID 18832067.
MCM9 binds Cdt1 and is required for the assembly of prereplication complexes. Lutzmann M, et al. Mol Cell, 2008 Jul 25. PMID 18657502.
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