|Application ||WB, E|
|Other Accession||NP_001137443, 9425, 12593 (mouse)|
|Predicted||Mouse, Rat, Pig, Dog, Cow|
|Calculated MW||66482 Da|
|Other Names||Chromodomain Y-like protein, CDY-like, 220.127.116.11, CDYL, CDYL1|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-CDYL Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a RE1-silencing transcription factor (REST) corepressor that facilitates histone-lysine N-methyltransferase EHMT2 recruitment and H3K9 dimethylation at REST target genes for repression. Required for chromatin targeting and maximal enzymatic activity of polycomb repressive complex 2 (PRC2); acts as a positive regulator of PRC2 activity by bridging the pre-existing histone H3K27me3 and newly recruited PRC2 on neighboring nucleosomes. Has histone acetyltransferase activity, with a preference for histone H4.|
|Tissue Location||Ubiquitous. Expressed at moderate levels in all tissues examined. Isoform 2 is the most abundantly expressed isoform.|
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Chromodomain Y is a primate-specific Y-chromosomal gene family expressed exclusively in the testis and implicated in infertility. Although the Y-linked genes are testis-specific, this autosomal gene is ubiquitously expressed. The Y-linked genes arose by retrotransposition of an mRNA from this gene, followed by amplification of the retroposed gene. Proteins encoded by this gene superfamily possess a chromodomain, a motif implicated in chromatin binding and gene suppression, and a catalytic domain believed to be involved in histone acetylation. Multiple proteins are encoded by transcript variants of this gene.
Multimerization and H3K9me3 binding are required for CDYL1b heterochromatin association. Franz H, et al. J Biol Chem, 2009 Dec 11. PMID 19808672.
The variant rs1867277 in FOXE1 gene confers thyroid cancer susceptibility through the recruitment of USF1/USF2 transcription factors. Landa I, et al. PLoS Genet, 2009 Sep. PMID 19730683.
CDYL bridges REST and histone methyltransferases for gene repression and suppression of cellular transformation. Mulligan P, et al. Mol Cell, 2008 Dec 5. PMID 19061646.
Phosphoproteome analysis of the human mitotic spindle. Nousiainen M, et al. Proc Natl Acad Sci U S A, 2006 Apr 4. PMID 16565220.
Binding of proteins to the PDZ domain regulates proteolytic activity of HtrA1 serine protease. Murwantoko, et al. Biochem J, 2004 Aug 1. PMID 15101818.
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