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Goat Anti-CENTB1 / ACAP1 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-CENTB1 / ACAP1 Antibody AF1228a
    AF1644a2 staining (0.5 µg/ml) of Jurkat lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15027
Other Accession NP_055531, 9744, 216859 (mouse)
Reactivity Human
Predicted Mouse, Rat, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 81536 Da
Additional Information
Gene ID 9744
Other Names Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1, Centaurin-beta-1, Cnt-b1, ACAP1, CENTB1, KIAA0050
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-CENTB1 / ACAP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACAP1
Synonyms CENTB1, KIAA0050
Function GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
Cellular Location Recycling endosome membrane; Peripheral membrane protein; Cytoplasmic side
Tissue Location Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.
Research Areas
Citations (0)

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Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.
Substrate specificities and activities of AZAP family Arf GAPs in vivo. Cuthbert EJ, et al. Am J Physiol Cell Physiol, 2008 Jan. PMID 18003747.
An ACAP1-containing clathrin coat complex for endocytic recycling. Li J, et al. J Cell Biol, 2007 Jul 30. PMID 17664335.
Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening. Wu C, et al. Proteomics, 2007 Jun. PMID 17474147.
Cooperation of phosphoinositides and BAR domain proteins in endosomal tubulation. Shinozaki-Narikawa N, et al. Traffic, 2006 Nov. PMID 17010122.

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$ 325.00
Cat# AF1228a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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