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Goat Anti-CHA / TCFL5 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-CHA / TCFL5 Antibody AF1234a
    AF1234a (0.3 µg/ml) staining of Human Testis lysate (35 µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UL49
Other Accession NP_006593, 10732, 277353 (mouse)
Reactivity Human
Predicted Mouse, Rat, Dog
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 52697 Da
Additional Information
Gene ID 10732
Other Names Transcription factor-like 5 protein, Cha transcription factor, HPV-16 E2-binding protein 1, E2BP-1, TCFL5, CHA, E2BP1
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-CHA / TCFL5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TCFL5
Synonyms CHA, E2BP1
Function Putative transcription factor. Isoform 3 may play a role in early spermatogenesis.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00981, ECO:0000269|PubMed:9763657}
Tissue Location Isoform 3 is testis specific. Isoform 2 is pancreas specific.
Research Areas
Citations (0)

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Regulation of TopBP1 oligomerization by Akt/PKB for cell survival. Liu K, et al. EMBO J, 2006 Oct 18. PMID 17006541.
Cha, a basic helix-loop-helix transcription factor involved in the regulation of upstream stimulatory factor activity. Rodr铆guez CI, et al. J Biol Chem, 2003 Oct 31. PMID 12923186.
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12477932.
The DNA sequence and comparative analysis of human chromosome 20. Deloukas P, et al. Nature, 2001 Dec 20-27. PMID 11780052.
Dominant T- and B-cell epitopes in an autoantigen linked to Chagas' disease. Giron脷s N, et al. J Clin Invest, 2001 Apr. PMID 11306602.

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$ 325.00
Cat# AF1234a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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