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Goat Anti-CHFR Antibody

Peptide-affinity purified goat antibody

  • IHC - Goat Anti-CHFR Antibody AF1236a
    AF1236a (2.5 µg/ml) staining of paraffin embedded human brain (cerebellum). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96EP1
Other Accession NP_001154819, 55743, 231600 (mouse), 288734 (rat)
Reactivity Human
Predicted Mouse, Rat
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 73386 Da
Additional Information
Gene ID 55743
Other Names E3 ubiquitin-protein ligase CHFR, 6.3.2.-, Checkpoint with forkhead and RING finger domains protein, RING finger protein 196, CHFR, RNF196
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-CHFR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms RNF196
Function E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.
Cellular Location Nucleus, PML body
Tissue Location Ubiquitous..
Research Areas
Citations (0)

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CHFR functions as a ubiquitin ligase for HLTF to regulate its stability and functions. Kim JM, et al. Biochem Biophys Res Commun, 2010 May 14. PMID 20388495.
Aberrant gene methylation in the peritoneal fluid is a risk factor predicting peritoneal recurrence in gastric cancer. Hiraki M, et al. World J Gastroenterol, 2010 Jan 21. PMID 20082478.
Aberrant promoter hypermethylation of the CHFR gene in oral squamous cell carcinomas. Baba S, et al. Oncol Rep, 2009 Nov. PMID 19787237.
CHFR promoter hypermethylation and reduced CHFR mRNA expression in ovarian cancer. Gao Y, et al. Int J Biol Markers, 2009 Apr-Jun. PMID 19634111.
Novel susceptibility loci for second primary tumors/recurrence in head and neck cancer patients: large-scale evaluation of genetic variants. Wu X, et al. Cancer Prev Res (Phila), 2009 Jul. PMID 19584075.

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$ 325.00
Cat# AF1236a
(40 western blots)
Availability: 7-10 days
Bulk Size
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