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Goat Anti-DAP3 Antibody

Peptide-affinity purified goat antibody

  • ICC - Goat Anti-DAP3 Antibody AF1300a
    AF1300a (0.5ug/ml) staining of guanidinium thiocyanate-treated HeLa before (left) and after (right) si-RNA-mediated DAP3 knock-down expresson. Primary incubation 1h at ambient temp. Detection by DyLight 488. Nuclear DAPI stain.
  • WB - Goat Anti-DAP3 Antibody AF1300a
    AF1300a (1 µg/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated DAP3 knock-down expresson in right lane) (35 µg protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence.
  • WB - Goat Anti-DAP3 Antibody AF1300a
    AF1300a staining (3 µg/ml) of HeLa lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • WB - Goat Anti-DAP3 Antibody AF1300a
    HEK293 overexpressing DAP3 (RC223182) and probed with AF1300a (mock transfection in first lane), tested by Origene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P51398
Other Accession NP_004623, 7818
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 45566 Da
Additional Information
Gene ID 7818
Other Names 28S ribosomal protein S29, mitochondrial, MRP-S29, S29mt, Death-associated protein 3, DAP-3, Ionizing radiation resistance conferring protein, DAP3, MRPS29
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-DAP3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DAP3
Synonyms MRPS29
Function Involved in mediating interferon-gamma-induced cell death.
Cellular Location Mitochondrion
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q.


IPS-1 is crucial for DAP3-mediated anoikis induction by caspase-8 activation. Li HM, et al. Cell Death Differ, 2009 Dec. PMID 19644511.
Death-associated protein 3 is overexpressed in human thyroid oncocytic tumours. Jacques C, et al. Br J Cancer, 2009 Jul 7. PMID 19536094.
hNOA1 interacts with complex I and DAP3 and regulates mitochondrial respiration and apoptosis. Tang T, et al. J Biol Chem, 2009 Feb 20. PMID 19103604.
Polymorphisms in mitochondrial genes and prostate cancer risk. Wang L, et al. Cancer Epidemiol Biomarkers Prev, 2008 Dec. PMID 19064571.
Identification of phosphorylation sites in mammalian mitochondrial ribosomal protein DAP3. Miller JL, et al. Protein Sci, 2008 Feb. PMID 18227431.

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$ 325.00
Cat# AF1300a
(40 western blots)
Availability: 7-10 days
Bulk Size
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