|Application ||WB, E|
|Other Accession||NP_060873, 55802, 75901 (mouse)|
|Calculated MW||63309 Da|
|Other Names||mRNA-decapping enzyme 1A, 3.-.-.-, Smad4-interacting transcriptional co-activator, Transcription factor SMIF, DCP1A, SMIF|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-DCP1A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7- methyl guanine cap structure from mRNA molecules, yielding a 5'- phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.|
|Cellular Location||Cytoplasm, P-body. Nucleus. Note=Co- localizes with NANOS3 in the processing bodies (By similarity) Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4.|
|Tissue Location||Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway.
Dcp1-bodies in mouse oocytes. Swetloff A, et al. Mol Biol Cell, 2009 Dec. PMID 19812249.
Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.
Human proline-rich nuclear receptor coregulatory protein 2 mediates an interaction between mRNA surveillance machinery and decapping complex. Cho H, et al. Mol Cell, 2009 Jan 16. PMID 19150429.
New application of intelligent agents in sporadic amyotrophic lateral sclerosis identifies unexpected specific genetic background. Penco S, et al. BMC Bioinformatics, 2008 May 30. PMID 18513389.
Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.
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