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Goat Anti-DGAT1 Antibody
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | O75907 |
| Other Accession | NP_036211, 8694, 13350 (mouse), 84497 (rat) |
| Reactivity | Human, Rat |
| Predicted | Mouse, Dog |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 100ug/200ul |
| Isotype | IgG |
| Calculated MW | 55278 Da |
| Gene ID | 8694 |
|---|---|
| Other Names | Diacylglycerol O-acyltransferase 1, 2.3.1.20, ACAT-related gene product 1, Acyl-CoA retinol O-fatty-acyltransferase, ARAT, Retinol O-fatty-acyltransferase, 2.3.1.76, Diglyceride acyltransferase, DGAT1, AGRP1, DGAT |
| Format | 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Goat Anti-DGAT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DGAT1 {ECO:0000303|PubMed:16214399, ECO:0000312|HGNC:HGNC:2843} |
|---|---|
| Function | Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates (PubMed:16214399, PubMed:18768481, PubMed:28420705, PubMed:9756920, PubMed:32433611, PubMed:32433610). Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats (PubMed:18768481). In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage (PubMed:16214399). Also present in female mammary glands, where it produces fat in the milk (By similarity). May be involved in VLDL (very low density lipoprotein) assembly (PubMed:18768481). In contrast to DGAT2 it is not essential for survival (By similarity). Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders (PubMed:16214399). Exhibits additional acyltransferase activities, includin acyl CoA:monoacylglycerol acyltransferase (MGAT), wax monoester and wax diester synthases (By similarity). Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed:28420705). |
| Cellular Location | Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9Z2A7}; Multi-pass membrane protein |
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Provided below are standard protocols that you may find useful for product applications.
Background
The enzyme encoded by this gene utilizes diacylglycerol and fatty acyl CoA as substrates in order to catalyze the final stage of triacylglycerol synthesis. It is also involved in cellular as well as physiological metabolic processes.
References
DGAT1 participates in the effect of HNF4A on hepatic secretion of triglyceride-rich lipoproteins. Krapivner S, et al. Arterioscler Thromb Vasc Biol, 2010 May. PMID 20167659.
Visceral and subcutaneous adipose tissue diacylglycerol acyltransferase activity in humans. Hou XG, et al. Obesity (Silver Spring), 2009 Jun. PMID 19197254.
Acylation of acylglycerols by acyl coenzyme A:diacylglycerol acyltransferase 1 (DGAT1). Functional importance of DGAT1 in the intestinal fat absorption. Cheng D, et al. J Biol Chem, 2008 Oct 31. PMID 18768481.
Thematic review series: glycerolipids. DGAT enzymes and triacylglycerol biosynthesis. Yen CL, et al. J Lipid Res, 2008 Nov. PMID 18757836.
AGPAT6 is a novel microsomal glycerol-3-phosphate acyltransferase. Chen YQ, et al. J Biol Chem, 2008 Apr 11. PMID 18238778.
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