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Goat Anti-DHX9 / RHA Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-DHX9 / RHA Antibody AF1317a
    AF1317a (1 µg/ml) staining of nuclear HeLa lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q08211
Other Accession NP_001348, 1660, 13211 (mouse), 304859 (rat)
Reactivity Human
Predicted Mouse, Rat, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 140958 Da
Additional Information
Gene ID 1660
Other Names ATP-dependent RNA helicase A, RHA,, DEAH box protein 9, Leukophysin, LKP, Nuclear DNA helicase II, NDH II, DHX9, DDX9, LKP, NDH2
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-DHX9 / RHA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DHX9
Synonyms DDX9, LKP, NDH2
Function Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD- mediated complex that promotes MYC mRNA stability. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms. Positively regulates HIV-1 LTR-directed gene expression.
Cellular Location Nucleus, nucleolus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm
Research Areas
Citations (0)

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This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.


Crystal structure of human RNA helicase A (DHX9): structural basis for unselective nucleotide base binding in a DEAD-box variant protein. Sch脤tz P, et al. J Mol Biol, 2010 Jul 23. PMID 20510246.
Kaposi's sarcoma-associated herpesvirus viral protein kinase interacts with RNA helicase a and regulates host gene expression. Jong JE, et al. J Microbiol, 2010 Apr. PMID 20437153.
RNA helicase A acts as a bridging factor linking nuclear beta-actin with RNA polymerase II. Tang W, et al. Biochem J, 2009 May 27. PMID 19309309.
An antiviral response directed by PKR phosphorylation of the RNA helicase A. Sadler AJ, et al. PLoS Pathog, 2009 Feb. PMID 19229320.
Genetic sequence variations of BRCA1-interacting genes AURKA, BAP1, BARD1 and DHX9 in French Canadian families with high risk of breast cancer. Gu茅nard F, et al. J Hum Genet, 2009 Mar. PMID 19197335.

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$ 325.00
Cat# AF1317a
(40 western blots)
Availability: 7-10 days
Bulk Size
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