|Application ||WB, E|
|Other Accession||NP_004411, 1850, 18218 (mouse)|
|Predicted||Mouse, Rat, Cow|
|Calculated MW||65827 Da|
|Other Names||Dual specificity protein phosphatase 8, 18.104.22.168, 22.214.171.124, Dual specificity protein phosphatase hVH-5, DUSP8, C11orf81, VH5|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-DUSP8 / HVH5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||This protein shows both activity toward tyrosine-protein phosphate as well as with serine/threonine-protein phosphate.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Abundant in brain, heart and skeletal muscle.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2.
Identification of a transcriptionally active hVH-5 pseudogene on 10q22.2. Berger IR, et al. Cancer Genet Cytogenet, 2005 Jun. PMID 15899389.
Investigation of DUSP8 and CALCA in alcohol dependence. Hink RL, et al. Addict Biol, 2003 Sep. PMID 13129832.
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12477932.
Genomic organization and chromosomal localization of a member of the MAP kinase phosphatase gene family to human chromosome 11p15.5 and a pseudogene to 10q11.2. Nesbit MA, et al. Genomics, 1997 Jun 1. PMID 9192849.
Normalization and subtraction: two approaches to facilitate gene discovery. Bonaldo MF, et al. Genome Res, 1996 Sep. PMID 8889548.
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