|Application ||WB, E|
|Other Accession||NP_055416, 30846|
|Predicted||Mouse, Rat, Dog, Cow|
|Calculated MW||61161 Da|
|Other Names||EH domain-containing protein 2, PAST homolog 2, EHD2, PAST2|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-EHD2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Required for translocation of FER1L5 to the plasma membrane. Binds ATP; does not bind GTP (By similarity).|
|Cellular Location||Cell membrane; Peripheral membrane protein; Cytoplasmic side. Endosome membrane; Peripheral membrane protein Note=Colocalizes with GLUT4 in intracellular tubulovesicular structures that are associated with cortical F-actin. Colocalizes with FER1L5 at plasma membrane in myoblasts and myotubes (By similarity).|
|Tissue Location||Highly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.
EH domain proteins regulate cardiac membrane protein targeting. Gudmundsson H, et al. Circ Res, 2010 Jul 9. PMID 20489164.
Architectural and mechanistic insights into an EHD ATPase involved in membrane remodelling. Daumke O, et al. Nature, 2007 Oct 3. PMID 17914359.
Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Kimura K, et al. Genome Res, 2006 Jan. PMID 16344560.
C-terminal EH-domain-containing proteins: consensus for a role in endocytic trafficking, EH? Naslavsky N, et al. J Cell Sci, 2005 Sep 15. PMID 16155252.
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.
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