Goat Anti-FBXW2 Antibody
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, E |
---|---|
Primary Accession | Q9UKT8 |
Other Accession | NP_036296, 26190 |
Reactivity | Human, Mouse, Rat |
Predicted | Pig, Dog, Cow |
Host | Goat |
Clonality | Polyclonal |
Concentration | 100ug/200ul |
Isotype | IgG |
Calculated MW | 51512 Da |
Gene ID | 26190 |
---|---|
Other Names | F-box/WD repeat-containing protein 2, F-box and WD-40 domain-containing protein 2, Protein MD6, FBXW2, FBW2, FWD2 |
Format | 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Goat Anti-FBXW2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | FBXW2 |
---|---|
Synonyms | FBW2, FWD2 |
Function | Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. |
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Provided below are standard protocols that you may find useful for product applications.
Background
F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. Mammalian F-box proteins are classified into three groups based on the presence of either WD-40 repeats, leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. This gene encodes the second identified member of the F-box gene family and contains multiple WD-40 repeats.
References
Ubiquitin-conjugating enzyme UBE2D2 is responsible for FBXW2 (F-box and WD repeat domain containing 2)-mediated human GCM1 (glial cell missing homolog 1) ubiquitination and degradation. Chiang MH, et al. Biol Reprod, 2008 Nov. PMID 18703417.
Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Kimura K, et al. Genome Res, 2006 Jan. PMID 16344560.
FBW2 targets GCMa to the ubiquitin-proteasome degradation system. Yang CS, et al. J Biol Chem, 2005 Mar 18. PMID 15640526.
M-phase kinases induce phospho-dependent ubiquitination of somatic Wee1 by SCFbeta-TrCP. Watanabe N, et al. Proc Natl Acad Sci U S A, 2004 Mar 30. PMID 15070733.
Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039.
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