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Goat Anti-FHL1 / SLIM1 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-FHL1 / SLIM1 Antibody AF1414a
    AF1414a staining (0.05 µg/ml) of Human Muscle lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • IHC - Goat Anti-FHL1 / SLIM1 Antibody AF1414a
    AF1414a (2.5 µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13642
Other Accession NP_001153171, 2273, 14199 (mouse), 25177 (rat)
Reactivity Human, Mouse
Predicted Rat, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 36263 Da
Additional Information
Gene ID 2273
Other Names Four and a half LIM domains protein 1, FHL-1, Skeletal muscle LIM-protein 1, SLIM, SLIM-1, FHL1, SLIM1
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-FHL1 / SLIM1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FHL1
Synonyms SLIM1
Function May have an involvement in muscle development or hypertrophy.
Cellular Location Isoform 1: Cytoplasm. Isoform 2: Nucleus. Cytoplasm, cytosol. Note=Predominantly nuclear in myoblasts but is cytosolic in differentiated myotubes
Tissue Location Isoform 1 is highly expressed in skeletal muscle and to a lesser extent in heart, placenta, ovary, prostate, testis, small intestine, colon and spleen. Expression is barely detectable in brain, lung, liver, kidney, pancreas, thymus and peripheral blood leukocytes. Isoform 2 is expressed in brain, skeletal muscle and to a lesser extent in heart, colon, prostate and small intestine. Isoform 3 is expressed in testis, heart and skeletal muscle.
Research Areas
Citations (0)

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This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.


Contractures and hypertrophic cardiomyopathy in a novel FHL1 mutation. Knoblauch H, et al. Ann Neurol, 2010 Jan. PMID 20186852.
Immune evasion of the human pathogenic yeast Candida albicans: Pra1 is a Factor H, FHL-1 and plasminogen binding surface protein. Luo S, et al. Mol Immunol, 2009 Dec. PMID 19850343.
Mutations of the FHL1 gene cause Emery-Dreifuss muscular dystrophy. Gueneau L, et al. Am J Hum Genet, 2009 Sep. PMID 19716112.
Consequences of mutations within the C terminus of the FHL1 gene. Schoser B, et al. Neurology, 2009 Aug 18. PMID 19687455.
Four and a half LIM domains 1 (FHL1) and receptor interacting protein of 140kDa (RIP140) interact and cooperate in estrogen signaling. Lin J, et al. Int J Biochem Cell Biol, 2009 Jul. PMID 19401155.

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$ 325.00
Cat# AF1414a
(40 western blots)
Availability: 7-10 days
Bulk Size
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