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Goat Anti-Flotillin 1 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-Flotillin 1 Antibody AF1421a
    AF1421a staining (0.3 µg/ml) of H460 lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • WB - Goat Anti-Flotillin 1 Antibody AF1421a
    HEK293 overexpressing FLOT1(RC200231) and probed with AF1421a (mock transfection in first lane), tested by Origene.
  • IHC - Goat Anti-Flotillin 1 Antibody AF1421a
    AF1421a (3.8 µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75955
Other Accession NP_005794, 10211, 14251 (mouse)
Reactivity Human
Predicted Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 47355 Da
Additional Information
Gene ID 10211
Other Names Flotillin-1, FLOT1
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-Flotillin 1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FLOT1
Function May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Cellular Location Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Melanosome Endosome. Note=Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Research Areas
Citations (0)

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Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.


Flotillins are involved in the polarization of primitive and mature hematopoietic cells. Rajendran L, et al. PLoS One, 2009 Dec 22. PMID 20027317.
High-density SNP screening of the major histocompatibility complex in systemic lupus erythematosus demonstrates strong evidence for independent susceptibility regions. Barcellos LF, et al. PLoS Genet, 2009 Oct. PMID 19851445.
The Mycobacterium bovis bacille Calmette-Guerin phagosome proteome. Lee BY, et al. Mol Cell Proteomics, 2010 Jan. PMID 19815536.
Flotillins interact with PSGL-1 in neutrophils and, upon stimulation, rapidly organize into membrane domains subsequently accumulating in the uropod. Rossy J, et al. PLoS One, 2009. PMID 19404397.
Protein delivery with transportans is mediated by caveolae rather than flotillin-dependent pathways. S脌脌lik P, et al. Bioconjug Chem, 2009 May 20. PMID 19348413.

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$ 325.00
Cat# AF1421a
(40 western blots)
Availability: 7-10 days
Bulk Size
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