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Goat Anti-Flotillin 2 / FLOT2 (C Term) Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-Flotillin 2 / FLOT2 (C Term) Antibody AF1422a
    AF1422a (0.1 µg/ml) staining of Human Brain (Cerebellum) lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14254
Other Accession NP_004466, 2319
Reactivity Human
Predicted Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 47064 Da
Additional Information
Gene ID 2319
Other Names Flotillin-2, Epidermal surface antigen, ESA, Membrane component chromosome 17 surface marker 1, FLOT2, ESA1, M17S1
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-Flotillin 2 / FLOT2 (C Term) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FLOT2
Synonyms ESA1, M17S1
Function May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
Cellular Location Cell membrane; Peripheral membrane protein Membrane, caveola; Peripheral membrane protein. Endosome. Note=Membrane-associated protein of caveolae
Tissue Location In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes
Research Areas
Citations (0)

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Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling.


Cellular roles of the prion protein in association with reggie/flotillin microdomains. Solis GP, et al. Front Biosci, 2010 Jun 1. PMID 20515742.
Flotillins are involved in the polarization of primitive and mature hematopoietic cells. Rajendran L, et al. PLoS One, 2009 Dec 22. PMID 20027317.
Basolateral internalization of GPI-anchored proteins occurs via a clathrin-independent flotillin-dependent pathway in polarized hepatic cells. A茂t-Slimane T, et al. Mol Biol Cell, 2009 Sep. PMID 19605558.
Paradoxical condensation of copper with elevated beta-amyloid in lipid rafts under cellular copper deficiency conditions: implications for Alzheimer disease. Hung YH, et al. J Biol Chem, 2009 Aug 14. PMID 19542222.
Flotillins interact with PSGL-1 in neutrophils and, upon stimulation, rapidly organize into membrane domains subsequently accumulating in the uropod. Rossy J, et al. PLoS One, 2009. PMID 19404397.

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$ 325.00
Cat# AF1422a
(40 western blots)
Availability: 7-10 days
Bulk Size
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