|Application ||WB, IHC, E|
|Other Accession||NP_001444, 2296, 17300 (mouse)|
|Reactivity||Human, Mouse, Zebrafish|
|Calculated MW||56789 Da|
|Other Names||Forkhead box protein C1, Forkhead-related protein FKHL7, Forkhead-related transcription factor 3, FREAC-3, FOXC1, FKHL7, FREAC3|
|Target/Specificity||Western blot: In transfected HEK293 transiently expressing full-length Human FOXC1 (myc and DYKDDDDK tagged), a band of approx. 75kDa was observed. No bands were observed in mock-transfected and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 0.5-1µg/ml. An anonymous customer found positive results in WB on Human breast cancer cells (T47D). IHC: In paraffin embedded Human Kidney shows nuclear staining in DCT and select nuclear staining in the glomerulus. Recommended concentration, 4-6µg/ml. Customer successfully used in IHC-P in mouse (periocular mesenchyme, cornea)|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-FOXC1 (C Term) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. Regulates FOXO1 through binding to a conserved element, 5'- GTAAACAAA-3' in its promoter region, implicating FOXC1 as an important regulator of cell viability and resistance to oxidative stress in the eye.|
|Tissue Location||Expressed in all tissues and cell lines examined.|
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Provided below are standard protocols that you may find useful for product applications.
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly.
Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.
A Large-scale genetic association study of esophageal adenocarcinoma risk. Liu CY, et al. Carcinogenesis, 2010 Jul. PMID 20453000.
FOXC1 is a potential prognostic biomarker with functional significance in basal-like breast cancer. Ray PS, et al. Cancer Res, 2010 May 15. PMID 20406990.
DNA methylation profiling in doxorubicin treated primary locally advanced breast tumours identifies novel genes associated with survival and treatment response. Dejeux E, et al. Mol Cancer, 2010 Mar 25. PMID 20338046.
Frequent aberrant DNA methylation of ABCB1, FOXC1, PPP2R2B and PTEN in ductal carcinoma in situ and early invasive breast cancer. Muggerud AA, et al. Breast Cancer Res, 2010. PMID 20056007.
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